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Figure 3. FGF2 reduces I/R-induced oxidative stress in ECs. (A) WB assay of skeletal muscle samples with HO-1, NQO1, and <t>SOD1.</t> (B) Quantification of HO-1, NQO1, and SOD1 proteins at the protein level from (A), normalised to GAPDH band density. (C) Skeletal muscle slices with SOD1 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (D) Quantification of SOD1 and CD31 cells with double positivity and percentages of total CD31 positive cells. (E) Images of skeletal muscle sections stained with DHE, nuclei were recognized by DAPI staining. Scale bars: 100 μm. Using the immunofluorescence data in (E), the average ROS optical density is shown in (F). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.
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Figure 3. FGF2 reduces I/R-induced oxidative stress in ECs. (A) WB assay of skeletal muscle samples with HO-1, NQO1, and <t>SOD1.</t> (B) Quantification of HO-1, NQO1, and SOD1 proteins at the protein level from (A), normalised to GAPDH band density. (C) Skeletal muscle slices with SOD1 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (D) Quantification of SOD1 and CD31 cells with double positivity and percentages of total CD31 positive cells. (E) Images of skeletal muscle sections stained with DHE, nuclei were recognized by DAPI staining. Scale bars: 100 μm. Using the immunofluorescence data in (E), the average ROS optical density is shown in (F). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.
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Image Search Results


Figure 3. FGF2 reduces I/R-induced oxidative stress in ECs. (A) WB assay of skeletal muscle samples with HO-1, NQO1, and SOD1. (B) Quantification of HO-1, NQO1, and SOD1 proteins at the protein level from (A), normalised to GAPDH band density. (C) Skeletal muscle slices with SOD1 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (D) Quantification of SOD1 and CD31 cells with double positivity and percentages of total CD31 positive cells. (E) Images of skeletal muscle sections stained with DHE, nuclei were recognized by DAPI staining. Scale bars: 100 μm. Using the immunofluorescence data in (E), the average ROS optical density is shown in (F). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.

Journal: International journal of biological sciences

Article Title: FGF2 Alleviates Microvascular Ischemia-Reperfusion Injury by KLF2-mediated Ferroptosis Inhibition and Antioxidant Responses.

doi: 10.7150/ijbs.85692

Figure Lengend Snippet: Figure 3. FGF2 reduces I/R-induced oxidative stress in ECs. (A) WB assay of skeletal muscle samples with HO-1, NQO1, and SOD1. (B) Quantification of HO-1, NQO1, and SOD1 proteins at the protein level from (A), normalised to GAPDH band density. (C) Skeletal muscle slices with SOD1 and CD31, an EC marker, stained. DAPI staining is used to identify the nuclei. Scale bars: 100 μm. (D) Quantification of SOD1 and CD31 cells with double positivity and percentages of total CD31 positive cells. (E) Images of skeletal muscle sections stained with DHE, nuclei were recognized by DAPI staining. Scale bars: 100 μm. Using the immunofluorescence data in (E), the average ROS optical density is shown in (F). Data are presented as mean ± SD (n = 3-5 per group). Significance: *P < 0.05.

Article Snippet: The following companies provided the specific primary antibodies that were needed: HO-1 (10701-1-AP), NQO1 (11451-1-AP), SOD1 (24127-1-AP), AMPK (66536-1-lg), NFE2L2 (16396-1-AP), GAPDH (60004-1-lg) and Histone-H3 (17168-1-AP) from Proteintech Group; SLC7A11 (DF12509) and KLF2 (DF13602) from Affinity; GPX4 (381958) from ZEN Bio; p-AMPK (2535) from abcam; HDAC5 (A0632) and p-HDAC5 (AP0202) from Abclonal; 4-HNE (MAB3249-SP) from R&D Systems.

Techniques: Marker, Staining, Immunofluorescence

Figure 5. Through increased NFE2L2 activity, FGF2 mitigates oxidative stress and ferroptosis. (A) WB examination of skeletal muscle samples. (B) Quantification of protein level of NFE2L2 from (A) with normalized to Histone 3 band density. (C) WB assay of skeletal muscle tissues reveals the presence of HO-1, SOD1, SLC7A11, 4-HNE, and GPX4.

Journal: International journal of biological sciences

Article Title: FGF2 Alleviates Microvascular Ischemia-Reperfusion Injury by KLF2-mediated Ferroptosis Inhibition and Antioxidant Responses.

doi: 10.7150/ijbs.85692

Figure Lengend Snippet: Figure 5. Through increased NFE2L2 activity, FGF2 mitigates oxidative stress and ferroptosis. (A) WB examination of skeletal muscle samples. (B) Quantification of protein level of NFE2L2 from (A) with normalized to Histone 3 band density. (C) WB assay of skeletal muscle tissues reveals the presence of HO-1, SOD1, SLC7A11, 4-HNE, and GPX4.

Article Snippet: The following companies provided the specific primary antibodies that were needed: HO-1 (10701-1-AP), NQO1 (11451-1-AP), SOD1 (24127-1-AP), AMPK (66536-1-lg), NFE2L2 (16396-1-AP), GAPDH (60004-1-lg) and Histone-H3 (17168-1-AP) from Proteintech Group; SLC7A11 (DF12509) and KLF2 (DF13602) from Affinity; GPX4 (381958) from ZEN Bio; p-AMPK (2535) from abcam; HDAC5 (A0632) and p-HDAC5 (AP0202) from Abclonal; 4-HNE (MAB3249-SP) from R&D Systems.

Techniques: Activity Assay

Figure 6. FGF2 reduces oxidative stress and ferroptosis via KLF2-NFE2L2 pathway in vivo. (A) Skeletal muscle specimens were used for the KLF2 and NFE2L2 WB assay. (B) Protein quantification of KLF2 and NFE2L2 from (A), normalised to GAPDH band density and histone 3 band density, respectively. (C) Skeletal muscle specimens showing HO-1, SOD1, SLC7A11, 4-HNE, and GPX4 on a WB assay. (D) Quantification of the GAPDH band density-normalized HO-1 and SOD1 protein levels from (C). (E) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (C) with normalized to GAPDH band density. (F) DAPI staining shows the nuclei in these micrographs of skeletal muscle slices that have been immunostained for KLF2 and CD31. Scale bars: 100 μm. (G) Micrographs of skeletal muscle slices with NFE2L2 and CD31 immunostaining; DAPI labelling shows

Journal: International journal of biological sciences

Article Title: FGF2 Alleviates Microvascular Ischemia-Reperfusion Injury by KLF2-mediated Ferroptosis Inhibition and Antioxidant Responses.

doi: 10.7150/ijbs.85692

Figure Lengend Snippet: Figure 6. FGF2 reduces oxidative stress and ferroptosis via KLF2-NFE2L2 pathway in vivo. (A) Skeletal muscle specimens were used for the KLF2 and NFE2L2 WB assay. (B) Protein quantification of KLF2 and NFE2L2 from (A), normalised to GAPDH band density and histone 3 band density, respectively. (C) Skeletal muscle specimens showing HO-1, SOD1, SLC7A11, 4-HNE, and GPX4 on a WB assay. (D) Quantification of the GAPDH band density-normalized HO-1 and SOD1 protein levels from (C). (E) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (C) with normalized to GAPDH band density. (F) DAPI staining shows the nuclei in these micrographs of skeletal muscle slices that have been immunostained for KLF2 and CD31. Scale bars: 100 μm. (G) Micrographs of skeletal muscle slices with NFE2L2 and CD31 immunostaining; DAPI labelling shows

Article Snippet: The following companies provided the specific primary antibodies that were needed: HO-1 (10701-1-AP), NQO1 (11451-1-AP), SOD1 (24127-1-AP), AMPK (66536-1-lg), NFE2L2 (16396-1-AP), GAPDH (60004-1-lg) and Histone-H3 (17168-1-AP) from Proteintech Group; SLC7A11 (DF12509) and KLF2 (DF13602) from Affinity; GPX4 (381958) from ZEN Bio; p-AMPK (2535) from abcam; HDAC5 (A0632) and p-HDAC5 (AP0202) from Abclonal; 4-HNE (MAB3249-SP) from R&D Systems.

Techniques: In Vivo, Staining, Immunostaining